Effects of Various Semen Extenders on Semen Parameters for the Purpose of Human Male Fertility Preservation

BACKGROUND: Cryopreservation enables semen to be preserved at subzero temperatures, usually at -196°C. There is a need in preparing good extender for the semen to be cryopreserved until use, especially in the field of assisted reproduction.

OBJECTIVE: To elucidate the apt extender for preserving both infertile and fertile samples for a minimum period and to check the post thaw results for various extenders used.

MATERIALS AND METHODS: A total of 103 samples were collected for this research, and after semen analysis the semen samples were categorized into oligospermia (n=20), oligoasthenospermia (n = 22), asthenospermia (n=24), normospermia (n=28), and control (n = 9).

RESULTS: The extender supplemented with various antioxidants yields better results when compared to all the other extenders in case of fertile and infertile samples.

CONCLUSION: Supplementing semen extender with antioxidants and various ingredients is the concern in designing an apt semen extender recipe. This research prescribes antioxidant extender (E4) to preserve the infertile and fertile semen samples for the purpose of research and also for doing assisted reproduction.