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Free Content Enhanced Metabolic Function of Human Hepatocytes Cryopreserved with Low Concentration Me2SO and Polyol Additives at –80°C

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The metabolic function of cryopreserved cells, in addition to cell viability after thawing, is an important parameter in any successful cryopreservation protocol. Dimethyl sulfoxide (Me2SO) is known to affect the differentiation of recovered cells. In this study, we report that sugars and sugar alcohols increases cell recovery, and also improves the metabolic function of human hepatocytes that are cryopreserved using low concentration Me2SO (5%). Three sugars (glucose, sucrose, and trehalose) and three sugar alcohols (xylitol, maltol, and sorbitol) have been tested. Cell viability after thaw and 24-h post-thaw attachment rate of cryopreserved human hepatocytes were assessed. Post-thaw metabolic activities (albumin, glucose, urea content) were measured, and cell proliferation was observed with inverted microscope. Cell viability, post-thaw attachment rate and metabolic activity of cryopreserved hepatocytes are enhanced by the addition of 0.4M sorbitol into 5% Me2SO solution. The study concludes that 5% Me2SO + 0.4M sorbitol can replace the 10% Me2SO method for cryopreservation of human hepatocytes at -80°C freezer. The new solution may reduce the side effects on the patients and improve the safety of using cryopreserved hepatocytes.
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Keywords: CRYOPROTECTANT; DIMETHYL SULFOXIDE; HUMAN HEPATOCYTES; SORBITAL

Document Type: Research Article

Publication date: 01 July 2013

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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