In the present study, Yam mosaic virus (YMV) could be efficiently eliminated by cryotherapy in Dioscorea opposita. Shoot apices were precultured for 16 h with 0.3 M sucrose, encapsulated in sodium alginate and dehydrated for 4 h prior to direct immersion in liquid nitrogen.
Up to 90% of the plants regenerated from cryopreserved shoot tips were YMV-free, whereas only 40% of those regenerated using meristem culture were YMV-free. YMV-free yam plantlets could be propagated in vitro through nodal stem culture, with sequential subculturing at 6-week intervals
on medium containing 0.5 mg 1-1 kinetin. The microtubers formed at the bottom and axil of the explants, incubated at 30°C after being chilled (4°C) for 3 months, could be sprouted successfully under in vivo conditions. Healthy plants were established without any damaging
symptoms of the virus. Thus, cryotherapy provides an alternative method for efficient elimination of yam viruses, and could be simultaneously used for long-term storage of yam germplasm and for the production of virus-free plants.
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IN VITRO PROPAGATION;
YAM MOSAIC VIRUS (YMV)
Document Type: Research Article
Publication date: 01 March 2013
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.