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Free Content Effect of "Ice Blockers" in Solutions for Vitrification of in Vitro Matured Ovine Oocytes

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Authors: Marco-Jimenez, Francisco; Berlinguer, Fiammetta; Leoni, Giovanni G.; Succu, Sara; Naitana, Salvatore

Source: Cryoletters, Volume 33, Number 1, January 2012, pp. 41-44(4)

Publisher: Cryoletters

Polymers have been used as a substitute for serum in vitrification solutions for embryos and oocytes. This study was designed to replace serum with defined commercial macromolecules in vitrification solution for in vitro matured ovine oocytes. Oocytes were cryopreserved in two vitrification solutions (16.5% ethylene glycol + 16.5% dimethyl sulphoxide) supplemented with 1% of SuperCool X-1000 and 1% SuperCool Z-1000 (Ice Blockers) or 20% foetal calf serum (FCS). After warming, oocytes viability and developmental potential after processing for in vitro embryo production were assessed. The number of viable oocytes (87.4% and 85.9%), cleaveage rates (21.4% and 19.6%) and blastocyst development rates (4.8% and 4.5%) were similar for Ice Blockers and FCS, respectively. On the basis of these findings, it may be concluded that combined use of Ice Blockers (SuperCool X-1000 and SuperCool Z-1000) as supplementation in vitrification solution offers similar results to serum for vitrification of in vitro matured ovine oocytes.

Keywords: CRYOTOP; OOCYTE; OVINE; SUPERCOOL X-1000; SUPERCOOL Z-1000; VITRIFICATION

Document Type: Research Article

Publication date: 01 January 2012

More about this publication?

  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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