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Free Content Can Thermal Expansion differences between Cryopreserved Tissue and Cryoprotective Agents alone cause Cracking?

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One of the limiting factors in large-scale cryopreservation is the formation of fractures. The prevalence of cracking in cryopreserved bulky tissues is frequently associated with temperature gradients, which lead to non-uniform thermal contraction of the tissue. With new cryoprotectants available, it may be possible to reduce temperature gradients to much lower levels, in which case other contributions to mechanical stress development and cracking will become more significant. One potential contributor to such stress is the difference in thermal expansion between tissue and the cryoprotectant. The current study addresses the role of thermal expansion mismatch by drawing upon recently obtained experimental data and engineering models for the development of thermal stresses. This question is addressed for the case of cryopreservation via vitrification (glass formation), for which crystal formation is avoided, and tissues and solutions gradually transition from fluid-like to solid-like response, as the viscosity increases with decreasing temperature.

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Keywords: CRACKING; CRYOPRESERVATION; MODELING; SOLID MECHANICS; VITRIFICATION

Document Type: Research Article

Publication date: 01 November 2009

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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