Limbal Explants from Cryopreserved Cadaver Human Corneas. Immunofluorescence and Light Microscopy of Epithelial Cells Growing in Culture
The result clearly showed that limbal tissue, subjected to cryopreservation and long lasting (up to 12 months) storage in liquid nitrogen, retains the capacity to be source of migrating and proliferating epithelial cells in vitro including the presumed adult stem cells and transient amplifying cells.
Keywords: CRYOPRESERVATION; IMMUNOFLUORESCENCE; LIGHT MICROSCOPY; LIMBAL EXPLANT CULTURE; P63; VIMENTIN
Document Type: Research Article
Publication date: 01 May 2009
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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