In vitro shoot-tips of three cultivars of tropical taro (Colocasia esculenta var. sculenta (L.) Schott) were successfully cryopreserved by vitrification. Different conditioning treatments were required for each of the cultivars, while the vitrification protocol was constant for all. For the cultivars E399 and CPUK, shoot-tips from three-month-old in vitro plants grown on solidified MS were preconditioned on MS with 0.3 M sucrose in the dark for 16 h at 25°C. For the cultivar TNS, donor plants were preconditioned on solid MS with 90 g L−1 sucrose for seven weeks before cryopreservation. For vitrification, the shoot-tips were loaded with a solution of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C, dehydrated with PVS2 for 12 min at 25°C and plunged in liquid nitrogen. Vials were warmed by rapid shaking in a water bath at 40°C for 1 min 30. Shoot-tips were rehydrated in liquid MS with 1.2 M sucrose for 15 min at 25°C then plated on recovery medium. Shoot-tips resumed growth within a week and developed into plantlets six to eight weeks later without any callus formation. The best mean recoveries for the three cultivars were 21, 29 and 30% for E399, CPUK and TNS, respectively. This protocol was evaluated with five other taro cultivars with no success. However, this study has shown that vitrification has potential for cryopreserving tropical taro.
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Keywords:
CRYOPRESERVATION;
PRECONDITIONING;
PRECULTURE;
SHOOT-TIPS;
TROPICAL TARO [COLOCASIA ESCULENTA VAR. ESCULENTA (L.) SCHOTT];
VITRIFICATION
Document Type: Research Article
Publication date:
01 May 2006
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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.