Donor Liver Preservation and Stimulation of Adhesion Molecules : Cold Preservation Initiates the Cascade
Methods: Needle biopsies taken during and after donor liver preservation were studied in 28 cases by immuno-histochemical methods. Human responder cell cultures were exposed to the effluent cold storage solution after liver preservation in a further 10 transplants.
Results: Increased ICAM-1 staining was seen at the end of cold storage compared to baseline (P<0.01), whilst reperfusion caused further increase (P<0.001). The storage solutions stimulated ICAM-1 and E-Selectin expression in responder cells in vitro as a bio-assay (P<0.01 in each case).
Conclusions: Liver harvesting and cold storage can initiate adhesion molecule expression even before reperfusion in the recipient.
Keywords: ADHESION MOLECULES; DONOR LIVER PRESERVATION; ICAM-1; STORAGE SOLUTION
Document Type: Research Article
Publication date: 01 November 2003
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
Receive new issue alert- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content