Step-Wise Refolding of Recombinant Proteins
Protein refolding is still on trial-and-error basis. Here we describe step-wise dialysis refolding, in which denaturant concentration is altered in step-wise fashion. This technology controls the folding pathway by adjusting the concentrations of the denaturant and other solvent additives to induce sequential folding or disulfide formation.
Keywords: Step-wise refolding; aggregation; antibody fragment; arginine; dialysis
Document Type: Research Article
Publication date: 01 April 2010
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