Developing of A Novel Clinical Kit for Assaying of Alpha-fetoprotein Based on Luminescent and Magnetic Nanoparticles
Alpha-fetoprotein (α-FP, AFP) was the only serological marker currently available for the detection of hepatocellular carcinoma (HCC). As a novel biological luminescent label, the favorable properties of water-soluble CdTe quantum dots (QDs) have advantages over conventional fluorescent materials. Meantime, the magnetic separation technique has widely been applied to various aspects in biotechnology in recent years. In this paper, CdTe QDs and dextran–Fe3O4 magnetic nanoparticles were both applied on immunoassay for the determination of AFP concentration. Firstly, a mouse anti-human AFP antibody (primary antibody) was immobilized on magnetic nanoparticles, which were used as solid support. Secondly, another mouse anti-human AFP antibody (secondary antibody) was attached to the surface of the CdTe QDs via electrostatic interaction. It was found that when the pH value was 6.0, the volume ratio of CdTe (3×10-4 mol/L) to antibody (2 mg/mL) was 50:3, and the reaction time was more than 10 min, the complex of the secondary antibody and CdTe was formed and the strongest PL intensity was obtained. Thirdly, the above two complexes were conjugated with a serial of AFP concentrations. Then the relationship between the PL intensity and the AFP concentration was determined and the calibration equation— Y=k(X-9.31)/V—was obtained. Finally, ten samples of human serum were tested based on this equation. The results, to a large extent, were consistent with those obtained by Time-resolved Fluorescence. More importantly, the current method could be applied in many other antibody-antigen systems.
No Supplementary Data
No Article Media
Document Type: Research Article
Publication date: 2013-08-01
More about this publication?
- Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.