Study of Bi-Enzyme Immobilization onto Layered Double Hydroxides Nanomaterials for Histamine Biosensor Application

In this work, we present the development of a hybrid biomembrane based on the immobilization of diamine oxidase (DAO) into LDH thin films for histamine detection. The LDHs preselected as host matrixes are: hydrotalcites (Mg₂Al(CO₃)_0.5(OH)₆), Iowaite (Mg₄Fe(OH)₁₀Cl) and hydrocalumite (Ca₂Al(OH)₆Cl). The immobilized probes were characterized by atomic force microscopy (AFM) and attenuated total reflection infrared spectroscopy (IR-ATR mode). The analysis of these results shows that the immobilization of DAO occurs with all type of selected LDH and is stable after a 7 day-immersion in phosphate buffer solution. The LDH incorporating magnesium or calcium divalent cations present high-quality surface topology for DAO immobilization and the ability to keep the enzyme in a well conformation for biogenic amines catabolism and histamine detection.