Biocapped Nanoparticles are More Bioactive to HeLa Cells than its Chemical Counterpart

In the present study we synthesized silver nanoparticles by conventional way of using citrate reduction as well as by green route synthesis using common bacteria Escherichia coli to demonstrate cytotoxicity and cell cycle regulation on cultured human HeLa cells. We employed cell morphology and apoptosis study, cell viability assay, cell cycle analysis and statistical analysis to compare the efficacy of two silver nanoparticles obtained from two different sources having same optical properties. Studies suggested that biocapped nanoparticles, which were synthesized using bacterial route, have more cell killing ability than its chemical counterpart. Furthermore, absence of nuclear fragmentation confirmed non-apoptotic cell death and FACS study supports the same along with additional information about the arrested HeLa cell growth at S-phase of cell cycle.