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Home / Journal of the American Association for Laboratory Animal Science, Volume 39, Number 2

Open Access Evaluation of an Anesthetic Regimen for Retroorbital Blood Collection from Mice

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Many Institutional Animal Care and Use Committees require anesthetization of mice for retroorbital blood collection procedures. Optimal anesthetic regimens should provide chemical restraint and adequate analgesia for a sufficient but otherwise minimal duration. This study was conducted to evaluate the effectiveness, safety, and practical utility of the general anesthetic combination of ketamine and medetomidine with and without the topical anesthetic proparacaine. In addition, we sought to evaluate the reversal of general anesthesia with atipamezole. The findings suggest that the combination of ketamine, medetomidine, proparacaine, and atipamezole is a safe and humane anesthetic regimen for retroorbital collection of blood samples from mice.

Document Type: Research Article

Affiliations: Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105

Publication date: 01 March 2000

More about this publication?

  • The Journal of the American Association for Laboratory Animal Science (JAALAS) serves as an official communication vehicle for the American Association for Laboratory Animal Science (AALAS). The journal includes a section of refereed articles and a section of AALAS association news. The mission of the refereed section of the journal is to disseminate high-quality, peer-reviewed information on animal biology, technology, facility operations, management, and compliance as relevant to the AALAS membership. JAALAS accepts research reports (data-based) or scholarly reports (literature-based), with the caveat that all articles, including solicited manuscripts, must include appropriate references and must undergo peer review.

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