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Open Access HEPES Removal by OHP Treatment

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HEPES is widely used in biochemistry and molecular biology research studies and applications as a buffer solution. However, it is difficult to remove from wastewater stream at the conclusion of work. FMC's patented OHP technology was proven in this study to be effective and efficient for HEPES treatment. The OHP process is based on Fenton's reagents. An experimental design methodology was employed to investigate the optimal conditions for total organic carbon (TOC) reduction and reagent efficiency. The TOC was reduced up to 98% and an efficiency of 80% was obtained in H2O2 basis. The results showed that in the current test ranges, the TOC reduction will increase with any parameters except pH. The reagent efficiency was dramatically improved by the increased temperatures, but adversely impacted by the increase in hydrogen peroxide volumes. There is a good correlation between the TOC levels and the biochemical oxygen demand (BOD) numbers.

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Document Type: Research Article

Publication date: 2008-02-01

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