Skip to main content

A Comparison of Rapid Bacterial Methods to U.S. EPA-Approved Methods for Biosolids

Buy Article:

$30.00 plus tax (Refund Policy)

Abstract:

ABSTRACT: 

The U.S. Environmental Protection Agency (U.S. EPA) specifies methods for testing microorganisms in biosolids, including U.S. EPA Methods 1680 and 1682 for fecal coliforms and Salmonella sp., respectively. However, these methods require 3 to 5 days to complete. For research or nonregulatory process monitoring purposes, it would be desirable to have a more rapid, less labor-intensive method of enumerating these organisms. The purpose of this study was to compare a modified Colilert with Quanti-Tray/2000 method to U.S. EPA Method 1680 for enumeration of fecal coliforms and E. coli , and to compare spread plating on xylose-lysine deoxycholate (XLD) supplemented with 10 μg/L novobiocin to U.S. EPA Method 1682 for Salmonella sp. in biosolids. These alternative enumeration methods require only 1 day to complete, using fewer costly materials. This limited study indicated that the more rapid methods provide results that are statistically comparable to U.S. EPA methods under most conditions and would be acceptable for rapid, nonregulatory data collection.

Keywords: Colilert; E. coli; Quanti-Tray; Salmonella; biosolids; fecal coliforms

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/106143013X13596524515942

Publication date: July 1, 2013

More about this publication?
  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • WEF Bookstore
  • ingentaconnect is not responsible for the content or availability of external websites
wef/wer/2013/00000085/00000007/art00007
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more