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Tissue-Specific Distribution and Activity of Glutathione S-Transferases as Biomarkers in Bivalve Shellfishes

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In order to find the relationship between tissue-specific expressions of glutathione S-transferases (GSTs) and their function in preventing effects of environmental toxicants, GSTs were primarily purified by GST-Sepharose 4B affinity chromatography from liver intestine, gill, mantle, and adductor muscle of Asaphis dichotoma, Amusium pleuronectes pleuronectes, and Atactodea striata. Determination of GSTs was also made in samples taken from the tissue muscle enshielding of A. dichotoma and A. striata . Dodecyl sulfate sodium salt polyacrylamide gel electrophoresis (SDS-PAGE) showed that each tissue has its own unique patterns of GSTs. Studies on tissue-specific activity toward 1-chloro-2,4-dinitrobenzene (CDNB), 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl), 4-nitrophthalic acid (4-NPA), and ethacrynic acid (ECA) indicated that GSTs from liver intestine exhibited the highest CDNB conjugation activity among all individual tissues from any of the three species sampled. Furthermore, liver intestine and its connected tissue (muscle enshielding) in these shellfishes accounted for more than 50% of the total detoxification toward the xenobiotic electrophile CDNB.

Keywords: biomarker; glutathione s-transferase (GSTs); purification; tissue-specific activity

Document Type: Research Article


Publication date: 2013-05-01

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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