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Distribution of Nitrosomonas-Related Ammonia-Oxidizing Bacteria and Nitrobacter-Related Nitrite-Oxidizing Bacteria in Two Full-Scale Biological Nutrient Removal Plants

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Abstract:

The dominant nitrifying bacterial communities and nitrification performance of two biological nutrient removal plants were evaluated. Fluorescent in situ hybridization was used to detect and quantify the dominant nitrifying bacteria and polymerase chain reaction; cloning and sequence analysis of 16S rRNA genes was done for phylogenetic analysis. Fluorescent in situ hybridization‐confocal scanning laser microscopy studies revealed the presence and dominance of Nitrosomonas-related ammonia-oxidizing bacteria (AOB) and Nitrobacter-related nitrite-oxidizing bacteria (NOB); however, a significant variation in AOB/NOB ratios was recorded. The plant with an overall higher AOB/NOB ratio (≥1.0) and dissolved oxygen concentration (1.8 to 2.5 mg/L) showed a higher nitrification rate. This study has also shown the co-existence and variation in phylogenetically diverse Nitrosomonas-related AOB and Nitrobacter-related NOB at these two plants. These dissimilar, distinct distribution patterns of nitrifying communities could be attributed to wastewater characteristics and the process configuration, which, in turn, would have also affected the nitrification performance of the systems.

Keywords: ammonia-oxidizing bacteria; biological nutrient removal; fluorescent in situ hybridization; nitrification; nitrite-oxidizing bacteria

Document Type: Research Article

DOI: https://doi.org/10.2175/106143013X13596524516022

Affiliations: Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban, 4000, South Africa, telephone: +27 31 373 2346; facsimile: +27 31 373 2777

Publication date: 2013-04-01

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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