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Open Access Molecular Methods in Biological Systems

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In 2009, molecular methods continue to be widely applied in different areas of environmental engineering research. Fingerprinting techniques such as denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and automated ribosomal intergenic spacer analysis (ARISA) continue to be popular tools to track differences in microbial community composition over time and space. Fluorescent in situ hybridization (FISH) of whole cells was also commonly employed to observe microbes of interest in ecological systems. Real time quantitative PCR methods appear to have become much more mainstream and were used to conduct comparative analyses of communities in different systems and over time. Several data analysis methods and software was proposed to analyze large and complex genomic data. In this review we chose to focus on new or significantly revised methods, novel applications of existing methods, and a few high quality reviews published in 2009, since the body of literature using molecular methods in environmental engineering has become very large.

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Keywords: FISH; MOLECULAR MICROBIAL ECOLOGY; PCR; TRFLP

Document Type: Research Article

Publication date: 01 January 2010

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    Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

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