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Nitrate Removal from Agricultural Infiltrate by Bioaugmented Free and Alginate Entrapped Cells

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A bench-scale sand column experiment was conducted to investigate nitrate removal from synthetic agricultural infiltrate by denitrifying bacterial cells entrapped in calcium alginate compared to free cells. The effects of methanol as a carbon source and cell loading were examined. Low (0 to 50%) nitrate removal was observed in both entrapped and free-cell columns without methanol supplement. In the presence of methanol, nitrate removals of 90 to 99% and 56 to 75% were obtained for entrapped and free-cell columns, respectively. Nitrate removal followed first-order kinetics. The entrapped-cell columns achieved higher nitrate removal than the free-cell columns because of less bacterial losses. Scanning electron microscopic images supported the nitrate removal results that the denitrifying bacteria proliferated in the entrapment matrix, and several nitrogen gas voids were produced from denitrification.

Keywords: Bioaugmentation; calcium alginate; cell loading; denitrification; sand column

Document Type: Research Article


Publication date: July 1, 2010

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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