Effect of Medium Components on the Production of a Biosurfactant from Candida tropicalis Applied to the Removal of Hydrophobic Contaminants in Soil
The influence of medium constituents on the production of biosurfactants by Candida tropicalis cultivated in waste frying oil was investigated according to a fractional factorial 25-1 design. The combined effect of the C/Ninorganic, C/Fe, C/Mg, and C/P ratios and yeast extract on surface tension reduction, biosurfactant yield, emulsification activity, and biomass were studied. The highest biosurfactant yield was reached when low C/Mg and low C/P ratio variables were combined, while the cell growth was favored by increasing the nitrogen concentration. The highest surface tension net decrease, on the other hand, was observed at low yeast extract concentration, low C/Fe, and high C/P ratios. Emulsification indices against lubrication and automobile waste oil of approximately 65 to 95% were observed. The crude biosurfactant produced in the medium—formulated with 2% waste frying oil, 0.067% NH4Cl, 0.025% MgSO4·7H2O, 0.067% KH2PO4, and 0.0026% FeCl3·6H2O—removed approximately 78 to 97% of the petroleum and motor oil adsorbed in sand samples.
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Document Type: Research Article
Publication date: 2010-05-01
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Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year. Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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