Open Access Molecular Methods in Biological Systems

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Abstract:

In 2008, molecular methods continue to be mainstream in environmental engineering research. Fingerprinting techniques such as denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analysis continue to be popular tools to track differences in microbial community composition over time and space. Fluorescent in situ hybridization (FISH) of whole cells was also commonly employed. Real time quantitative PCR methods appear to have become much more mainstream and were used to conduct comparative analyses of communities in different systems and over time. In this review we chose to focus on new or significantly revised methods, novel applications of existing methods, and a few high quality reviews published in 2008, since the body of literature using molecular methods in environmental engineering has become very large.

Keywords: MOLECULAR MICROBIAL ECOLOGY; PCR

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/106143009X12445568399370

Publication date: September 10, 2009

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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