Effective Removal of Cryptosporidium by a Novel Bioflocculant
Abstract:Extracellular biopolymer produced from Klebsiella terrigena was found to have excellent flocculating ability over a wide range of colloid particles (0.5 to 25μ). The biopolymer was thermostable, with an optimum temperature for flocculation of 30°C. Analysis with Fourier transform infrared spectrophotometry (FT-IR) shows that the biopolymer mainly possesses hydroxyl, carboxyl, and methoxyl groups, with neutral sugar and uronic acid as its major and minor components, and the structure of a polysaccharide. The average molecular weight of the biopolymer was greater than 2 × 10 3 kilodalton (KDa), as determined by gel permeation chromatography. Scanning electron microscopy indicated a porous morphology of the biopolymer. At a dosage of 2 mg/L, the purified biopolymer could remove 62.3% of Cryptosporidium oocysts (1 × 10 6) spiked in tap water samples. Calcium (5mM) was required for effective removal. The removal efficiency of Cryptosporidium oocysts by the biopolymer remained unaltered over a pH range of 6 to 8. The results of this study indicates a possible utility of the Klebsiella terrigena biopolymer as an alternative to typically used chemical flocculants for removal of Cryptosporidium oocysts from water.
Document Type: Research Article
Publication date: February 1, 2009
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