Effective Removal of Cryptosporidium by a Novel Bioflocculant
Abstract:Extracellular biopolymer produced from Klebsiella terrigena was found to have excellent flocculating ability over a wide range of colloid particles (0.5 to 25μ). The biopolymer was thermostable, with an optimum temperature for flocculation of 30°C. Analysis with Fourier transform infrared spectrophotometry (FT-IR) shows that the biopolymer mainly possesses hydroxyl, carboxyl, and methoxyl groups, with neutral sugar and uronic acid as its major and minor components, and the structure of a polysaccharide. The average molecular weight of the biopolymer was greater than 2 × 10 3 kilodalton (KDa), as determined by gel permeation chromatography. Scanning electron microscopy indicated a porous morphology of the biopolymer. At a dosage of 2 mg/L, the purified biopolymer could remove 62.3% of Cryptosporidium oocysts (1 × 10 6) spiked in tap water samples. Calcium (5mM) was required for effective removal. The removal efficiency of Cryptosporidium oocysts by the biopolymer remained unaltered over a pH range of 6 to 8. The results of this study indicates a possible utility of the Klebsiella terrigena biopolymer as an alternative to typically used chemical flocculants for removal of Cryptosporidium oocysts from water.
Document Type: Research Article
Publication date: February 1, 2009
More about this publication?
- Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year. Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- WEF Bookstore
- Ingenta Connect is not responsible for the content or availability of external websites