@article {Atli:2007:1061-4303:958,
	author = "Atli, G and Canli, M",
	title = "Natural Occurrence of Metallothioneinlike Proteins in Liver Tissues of Four Fish Species from the Northeast Mediterranean Sea",
	journal = "Water Environment Research",
	volume = "79",
	number = "9",
	year = "2007",
	abstract = "Fish with different ecological needs were captured from the northeast Mediterranean Sea to determine metallothioneinlike proteins in their livers. Heat-treated liver samples were run on a column packed with Sephadex G-75 (Sigma, Germany) for subsequent determination of metals, sulfhydryl, and absorbance at 254 and 280 nm. Liver cytosols of Sparus auratus and Mullus barbatus had three heat-stable protein peaks, whereas Mugil cephalus and Atherina hepsetus had two. The third protein peak, containing metallothioneinlike proteins (5000 to 6000 Da), was present in all fish. Only high- and low-molecular-weight proteins contained sulfhydryl and metals. Zinc was the most abundant metal in the metallothioneinlike proteins, whereas cadmium and lead were not detected. The differences in the characteristics of metal-binding proteins among fish indicate that the biological characteristics of fish species should be carefully taken into account concerning natural monitoring studies.",
	pages = "958-963",
	url = "http://www.ingentaconnect.com/content/wef/wer/2007/00000079/00000009/art00004",
	doi = "doi:10.2175/106143007X175780",
	keyword = "fish, metal, metallothionein, biomarker"
}