Skip to main content

Natural Occurrence of Metallothioneinlike Proteins in Liver Tissues of Four Fish Species from the Northeast Mediterranean Sea

Buy Article:

$30.00 plus tax (Refund Policy)


Fish with different ecological needs were captured from the northeast Mediterranean Sea to determine metallothioneinlike proteins in their livers. Heat-treated liver samples were run on a column packed with Sephadex G-75 (Sigma, Germany) for subsequent determination of metals, sulfhydryl, and absorbance at 254 and 280 nm. Liver cytosols of Sparus auratus and Mullus barbatus had three heat-stable protein peaks, whereas Mugil cephalus and Atherina hepsetus had two. The third protein peak, containing metallothioneinlike proteins (5000 to 6000 Da), was present in all fish. Only high- and low-molecular-weight proteins contained sulfhydryl and metals. Zinc was the most abundant metal in the metallothioneinlike proteins, whereas cadmium and lead were not detected. The differences in the characteristics of metal-binding proteins among fish indicate that the biological characteristics of fish species should be carefully taken into account concerning natural monitoring studies.

Keywords: biomarker; fish; metal; metallothionein

Document Type: Research Article


Publication date: September 1, 2007

More about this publication?
  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • WEF Bookstore
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Partial Open Access Content
Partial Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more