Two most probable number (MPN) methods—lauryl tryptose broth with Escherichia coli broth confirmation and direct A-1 broth incubation (A-1)—were compared for the enumeration of fecal coliform in lime-treated biosolid. Fecal coliform numbers were significantly higher using
the A-1 method. Analysis of positive A-1 tubes, however, indicated that a high percentage of these were false positives. Therefore, the use of A-1 broth for 40 CFR Part 503 Pathogen Reduction (CFR, 1993) compliance testing is not recommended.
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