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Nitrifying Biofilm Development with Time: Activity Versus Phylogenetic Composition

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The development of nitrifying biofilms collected from a full-scale nitrifying trickling filter was evaluated through the application of fluorescent in situ hybridization (FISH) and by quantification of nitrification rates in bench-scale reactors. Two sampling campaigns were conducted to evaluate the structure and function of biofilms between 14 and 70 days old. The structure, or number of ammonia-oxidizing bacteria, was quantified with Nso190. The function was quantified with bench-scale nitrification rates. The two assays were compared by calculating correlation coefficients by simple linear regression of the two data sets. The number of ammonia-oxidizing bacteria closely tracked activity data (linear correlation, r 2 > 0.500). Changes in ammonia-oxidation capacity with time (7-day intervals) were mirrored by shifts in the percent of ammonia-oxidizing bacteria present. Nitrification rates did not correlate to the EUB338-probe stained area (r 2 < 0.500), suggesting that nonnitrifying bacteria play a larger role in nitrifying biofilm ecology than previously thought. Dry-weight biomass accumulations did not correlate to either the EUB338-probe stained area or the bench-scale nitrification rates. This suggests that inert materials accumulate in the biofilms over time.
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Document Type: Research Article

Publication date: 2002-09-01

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    Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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