@article {Zilles:September/October 2002:1061-4303:428,
	author = "Zilles, Julie L.",
	author = "Peccia, Jordan",
	author = "Noguera, Daniel R.",
	title = "Microbiology of Enhanced Biological Phosphorus Removal in AeratedAnoxic Orbal Processes",
	journal = "Water Environment Research",
	volume = "74",
	year = "September/October 2002",
	abstract = "The traditional process for enhanced biological phosphorus removal (EBPR) in wastewater treatment involves an anaerobic zone followed by an aerobic zone. Although there is no strict anaerobic zone in aerated-anoxic Orbal processes, phosphorus removal in excess of that required for cell growth does occur. The microbial ecology of polyphosphate-accumulating organisms (PAO) in two full-scale Orbal wastewater treatment plants was investigated using flow cytometry to physically separate PAO from non-PAO and fluorescent in situ hybridization (FISH) to identify organisms. Although <I>Candidatus Accumulibacter phosphatis</I>, an uncultured organism associated with EBPR in acetate-fed laboratory-scale reactors, was detected, it did not seem to be the dominant PAO in these processes. Comparative FISH analyses of the activated sludge and the PAO-rich subpopulation did not reveal the presence of a dominant group of PAO in these full-scale plants. Rather, the analysis suggested that the operational characteristics of aerated-anoxic processes might select for a diverse PAO community that is significantly different from that observed in acetate-fed laboratory reactors or in traditional EBPR configurations.",
	pages = "428-436(9)",
	url = "http://www.ingentaconnect.com/content/wef/wer/2002/00000074/00000005/art00002"
	doi = "doi:10.2175/106143002X140224"
}