If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Comparison of Various Measures of Microbial Growth Kinetics in Suspended and Biofilm Cultures During Biodegradation of Naphthalene

$30.00 plus tax (Refund Policy)

Buy Article:


This research focused on measurement of bacterial growth rates and activities in suspended cultures and biofilms using Pseudomonas putida (ATCC 17484) for biodegradation of naphthalene. A comparison was made of the effect of three biomass measures: optical density, total cell protein, and living cell number on the calculated rate of naphthalene disappearance. Living cell number was determined by optimizing a fluorescent staining technique and using epifluorescence microscopy. All three techniques gave similar results in terms of the specific growth rates, and in all three there was a lag time between the disappearance of naphthalene and the appearance of biomass. This was most likely a consequence of the production of intermediate products detected in the chromatograms. Inclusion of a one-hour lag time in the integrated Monod expression considerably improved the agreement between experimental and calculated values. However, further improvements to an understanding of the overall rate processes may require more detailed kinetics of the individual biochemical pathways.
More about this publication?
  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • WEF Bookstore
  • ingentaconnect is not responsible for the content or availability of external websites



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more