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Evaluation of Colilert and Enterolert Defined Substrate Methodology for Wastewater Applications

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Abstract:

This study evaluated the utility of defined substrate methodology (DSM) for the enumeration of indicator bacteria in wastewater applications. Two commercial products, Colilert and Enterolert systems (Idexx Laboratories, Westbrook, Maine), were evaluated for variation, false-positive results, and method correlation in both surface waters and treated wastewater effluent. The DSM tests performed as well or better than the traditional methodology. The Colilert total coliform test was also evaluated for its utility in estimating fecal coliforms by incubation at 44.5 ± 0.2 °C. The modified DSM total coliform test correlated well to membrane filtration (Pearson correlation coefficient 0.8) for two different sample groups, demonstrating its utility as a screening tool to estimate fecal coliform densities.

Keywords: COLILERT TOTAL COLIFORM TEST; DEFINED SUBSTRATE METHODOLOGY; ENTEROLERT ENTEROCOCCUS GROUP TEST; MEMBRANE FILTRATION; THERMOTOLERANT COLIFORMS

Document Type: Research Article

DOI: https://doi.org/10.2175/106143002X139839

Publication date: 2002-03-01

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  • Water Environment Research (WER) is published monthly, including an annual Literature Review. A subscription to WER includes access to the latest content back to 1992, as well as access to fast track articles. An individual subscription is valid for 12 months from month of purchase.

    Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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