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Size-Fractionated Alkaline Phosphatase Activity in the Hypereutrophic Villerest Reservoir (Roanne, France)

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Seasonal changes in size-fractionated alkaline phosphatase activity (APA) were studied in the hypereutrophic Villerest reservoir (Roanne, France) to determine whether the APA showed seasonal variations coupled to abiotic and biotic variables. Samples were collected at different depths from November 1992 to October 1993. The phosphatase activities were measured spectrophotometrically with p nitrophenyl phosphate as the substrate and related to protein concentrations. No correlations were found between APA and soluble reactive phosphorus concentrations during an annual cycle. Free phosphatases were particularly high in the epilimnion (54.5% of total activity was at 5 m) and decreased with depth (4% of total APA at 40 m). The contribution of small microorganisms to APA increased with depth. It was concluded that APA is not a valid quantitative test of the phosphorus status of aquatic microorganisms.


Document Type: Research Article


Publication date: March 1, 2001

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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