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Biodegradation kinetics of chlorophenols in immobilized-cell reactors using a white-rot fungus on wood chips

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Abstract:

This research investigated the ability of wood-chip reactors seeded with a white-rot fungus (Phanerochaete chrysosporium) to degrade hazardous substances (4-chlorophenol [4-CP] and 2,4-dichlorophenol [2,4-DCP]). Batch-reactor tests were conducted using 4-CP as a model compound to evaluate the effect of carbon and nitrogen deficiencies on the ability of white-rot fungus immobilized on wood chips to degrade 4-CP. The white-rot fungus degraded 4-CP (71.1 to 83.0%) under all tested conditions including the non-glucose and non-nitrogen conditions. However, there are differences in the degradation percentage of 4-CP using the different growing conditions. The degradation of 4-CP occurs to the greatest extent in the non-glucose/with-nitrogen condition (15.38 ppm/h·g of specific biodegradation rate). Continuous-flow packed-bed reactor tests are conducted using 2,4-DCP as a model compound to evaluate the inhibition effect of 2,4-DCP on the biodegradation enzymes in wood-chip reactor systems, and the inhibition effects seem to be present. The inhibition kinetics of 2,4-DCP are successfully modeled with the mass-balance equation of plug-flow reactors and a substrate-inhibition equation for the reaction rate, yielding an inhibition constant,Ki of 69.8 ppm and a maximum 2,4-DCP concentration, [S]max, of 48.9 ppm at the highest reaction rate. The importance of these results is that the substrate-inhibition model can be used to explain the inhibition effect of 2,4-DCP on the biodegradation enzymes in this wood-chip reactor system. This study points to the potential of continuous-flow reactors using wood chips as a carbon source to degrade toxic chemicals with high-degradation efficiency.

Keywords: 2,4-DICHLOROPHENOL; 4-CHLOROPHENOL; CONTINUOUS-FLOW PACKED-BED REACTOR; SUBSTRATE-INHIBITION MODEL; WHITE-ROT FUNGUS; WOOD-CHIP REACTOR

Document Type: Research Article

DOI: https://doi.org/10.2175/106143098X127053

Publication date: 1998-03-01

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    Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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