Performance of a continuous stirred tank reactor with immobilized denitrifiers and methanogens

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Water denitrification by a coimmobilized mixed culture of denitrifiers and methanogens to simultaneously remove nitrate and excessive carbon source, methanol, in a continuous stirred tank reactor was investigated. Complete denitrification occurred at a methanol to nitrate nitrogen (M:N) ratio of ≥2.62. Ratios beyond 2.62 methane formation took place resulting in the depletion of excess methanol. The quality of the effluent solution was found to be markedly dependent on nitrate loading. At a constant M:N ratio ∼3.2, a complete nitrate and methanol removal could be attained when the influent nitrate concentration was <385 mg N/L; otherwise, the hydraulic retention time (HRT) was operated >2 hr (at a constant influent nitrate around 100 mg N/L). The surplus methanol was verified to be converted directly into methane when complete denitrification was achieved. Moreover, a sufficient correlation was found between the biological activity and the redox potential.


Document Type: Research Article


Publication date: March 1, 1997

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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