Effects of temperature and pH on the kinetic growth of unialga Chlorella vulgaris cultures containing bacteria
Abstract:The kinetic growth of unialga Chlorella vulgaris was investigated in mixed-culture fed-batch chemosat reactors, with special reference to temperature and pH. The experiments were carried out at 10°C to 40°C, pH was controlled from 3.0 to 11.5 in a series of reactors, and glucose at a concentration of 75 mg/L was fed as the sole source of organic carbon. The maximum growth rate of 0.50 day−1 was obtained at pH of about 6.31 to 6.84, and the optimum temperature was 32.4°C. The activation energy of the growth-limiting reaction and the enthalpy change for the enzyme-inactivation reaction were 24.5 and 253.36 kJ/mole, respectively. The saturation constants for hydrogen ion, KH, and hydroxyl ion, KOH, were temperature-dependent. At temperatures below optimum, KH increased when temperature increased, but KOH decreased. Above the optimum temperature, KH decreased and KOH increased with temperature.
Document Type: Research Article
Publication date: 1997-01-01
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