Phase-separated anaerobic toxicity assays for sulfate and sulfide
Abstract:As compared with anaerobic toxicity assays (ATA) for industrial toxicants focused only on the acetoclastic methanogens, two phase-separated ATAs for sulfate and sulfide have been conducted by means of acidogenic and methanogenic cultures. Seed sludge was obtained from a two-phase upflow anaerobic sludge blanket (UASB) reactor which operated on glucose as a substrate. The obtained results were compared with those of mixed culture. In the acidogenic ATA, the glucose utilization rate could be expressed in a first-order reaction kinetic. Gas production and substrate utilization were affected slightly when sulfate concentration was up to 6 000 mg SO4-S/l. However, the substrate conversion to acetate was retarded by sulfate addition. When sulfide over 800 mgS/l was added in test bottles, glucose utilization rate was retarded and the first-order kinetic constant decreased. From the results of the methanogenic ATA, it was found that methanogens were more sensitive than acidogens as the sulfate and sulfide concentrations increased. Although methane production was slightly retarded by added sulfate, methane was produced up to 60% of the control when the sulfate was 6 000 mg SO4-S/l. Methane production was not inhibited up to 200 mgS/l; however, it was severely retarded at 400 mg S/l. The methane production was completely inhibited in excess of 800 mg S/l. In mixed culture, the inhibiting effects of sulfate and sulfide on mixed culture differed from those of phase-separated anaerobes. Methane production was only slightly inhibited when sulfate and sulfide concentrations reached up to 4 000 mg SO4-S/l and 1 200 mg S/l, respectively.
Document Type: Research Article
Publication date: July 1, 1995
More about this publication?
- Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year. Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- WEF Bookstore
- Ingenta Connect is not responsible for the content or availability of external websites