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Enhanced biodegradation of carbon tetrachloride by the supplement of substrate and mineral ions under anaerobic condition

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The effect of substrate and mineral elements on the anaerobic bioremediation of carbon tetrachloride (CT) was investigated by introducing different substrates and mineral elements into the microbial communities. Glucose, acetate, and humic acid were used as carbon and energy sources for microbial use, ferrous ion was used as the reducing agent, and nitrate was used as the electron acceptor.

Experimental results showed that the addition of substrates and mineral ions could enhance the biotransformation efficiency of CT. When follow-up substrates were delivered into the microcosms, 45% to 80% and 18% to 49% enhancement of CT removal were observed for glucose- and acetate-fed batches. This study indicated that the bioremediation of groundwater contaminated with heavily chlorinated hydrocarbons is feasible by applying the proper supplemental substrate and nutrients to the aquifer system with appropriate concentrations.


Document Type: Research Article


Publication date: May 1, 1995

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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