The effect of substrate and mineral elements on the anaerobic bioremediation of carbon tetrachloride (CT) was investigated by introducing different substrates and mineral elements into the microbial communities. Glucose, acetate, and humic acid were used as carbon and energy sources
for microbial use, ferrous ion was used as the reducing agent, and nitrate was used as the electron acceptor. Experimental results showed that the addition of substrates and mineral ions could enhance the biotransformation efficiency of CT. When follow-up substrates were delivered into
the microcosms, 45% to 80% and 18% to 49% enhancement of CT removal were observed for glucose- and acetate-fed batches. This study indicated that the bioremediation of groundwater contaminated with heavily chlorinated hydrocarbons is feasible by applying the proper supplemental substrate and
nutrients to the aquifer system with appropriate concentrations.
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