The loss of plasmid-encoded phenotypes in alcaligenes eutrophus, staphylococcus aureus, and pseudomonas putida during freeze-drying and storage

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Abstract:

Three bacterial strains, which serve as hosts for plasmids containing phenotypes of environmental interest, were subjected to freeze-drying and storage. Plasmid-encoded phenotypes investigated included heavy metal resistance in A. eutrophus and S. aureus and catechol 2,3-oxygenase enzyme activity in A. eutrophus and P. putida Both freeze drying and storage treatments were found to affect strain viability and plasmid phenotype expression. In all cases studied, loss of strain viability was greater than 95.6% after freeze-drying. Strain viability losses were accompanied by phenotype losses which averaged an order of magnitude greater than viability losses. Viability and phenotype loss continued during storage and were described using first-order decay. The implications of these trends for application of specialized cultures to promote enhanced biodegradation are discussed.

Keywords: BIOAUGMENTATION; FREEZE-DRYING; PLASMIDS; VIABILITY

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/106143095X131394

Publication date: March 1, 1995

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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