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The effects of freeze-drying and storage on phenol degradation

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Freeze-drying and storage were found to affect the performance of a microbial supplement cultured for phenol biodegradation. After freeze-drying, a lag in phenol degradation was observed which increased with storage. The lag was attributed to reductions in culture viability and lags in enzyme activity, both of which increased with storage. In addition, a switch in the primary pathway for aromatic ring cleavage from catechol 2,3-oxygenase to catechol 1,2-oxygenase occurred after freeze-drying. While freeze-drying and storage adversely affected viability and resulted in both a switch and lag in enzyme activity, increases in the maximum specific growth constant, µm, also were observed with this treatment. Both the observed lag in phenol degradation and increases in µm were simulated numerically.

Keywords: bioaugmentation; freeze-drying; kinetics; phenol; simulation; viability

Document Type: Research Article


Publication date: July 1, 1994

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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