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Multilevel protocol for assessing the fate and effect of toxic organic chemicals in anaerobic treatment processes

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A multilevel protocol has been developed to provide a consistent and repeatable means of determining fate and effect of toxic organic chemicals in anaerobic wastewater treatment processes. Level I consists of a three-step screening protocol for assessing toxicant effect on organic cosubstrate transformations. Level II is a cosubstrate kinetics protocol designed to reveal the effect of toxic organic chemicals on the kinetics of acetogenic and methanogenic transformations. Level III is a toxicant degradation kinetics protocol. The protocol test approach represents a composite of conditions and procedures used by a number of investigators of anaerobic reactions. It permits a wide latitude in specific reactor sizes and types and in measurement techniques as long as the basic culture, chemical and physical environment, and substrates are maintained as prescribed. Examples are presented to illustrate the type of test results that can be expected when using the protocol.

Keywords: anaerobic processes; biodegradation; fate and effect; kinetics; protocol; toxicant degradation; toxicity assessment

Document Type: Research Article


Publication date: January 1, 1993

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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