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Effluent toxicity testing using nitrifiers and Microtox™

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In an effort to develop a less expensive method of assessing wastewater toxicity, two bacterial alternatives to Ceriodaphnia were tested: the commercially available Microtox™ procedure (using Photobacterium phosphoreum) and a nitrifier respiration procedure. Forty-four biologically treated municipal wastewaters were evaluated. Neither the Microtox nor the nitrifier test is sufficiently sensitive to detect acute Ceriodaphnia toxicity when testing biologically treated municipal wastewaters. The 7-day chronic Ceriodaphnia procedure is more sensitive than the 48-hour acute Ceriodaphnia test, with 14 of the 16 wastewaters tested for chronic toxicity exhibiting toxicity using 100% wastewater.

Keywords: Ceriodaphnia; Microtox™; Photobacterium; bioassay; effluent; inhibition; nitrifiers; respirometry; toxicity

Document Type: Research Article


Publication date: May 1, 1992

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  • Water Environment Research® (WER®) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
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