Reintroduction of Contaminant in Groundwater Alters Bacterial Community
Bioaugmentation, a form of bioremediation, involves addition of microbial population acclimated with the contaminant of concern to degrade contaminants in situ. During bioremediation, periodic absence of the contaminant can alter the microbial community resulting in less efficient degradation. This study investigates the effects of supplying an alternate carbon source during the contaminant absence periods and/or bacterial degrader after the absence periods on the diversity of the bacterial community and the contaminant removal efficiency when the contaminant is reintroduced. Benzene being a common groundwater contaminant was used as the model contaminant in this study. Sand columns were set up to simulate groundwater flow and benzene breakthrough experiments were conducted to investigate benzene removal efficiencies (BREs) and bacterial community changes. Two benzene absence periods (BAP) of 10 or 25 days were tested during which only deionized water with no organic carbon source or ethanol was supplied. The effect of the absence period on the removal efficiency was more prominent during the longer absence period. Columns that received bacteria immediately after the absence periods showed superior performances than those that did not. A strain with 100% identity to Pseudomonas putida disappeared during the BAP but reemerged following the reintroduction of benzene. Columns provided with an alternative carbon source (ethanol) during the BAP and/or bacterial culture helped in maintaining the bacterial community and improving the BRE. This work identified changes in the bacterial community and their responses due to contaminant absence periods.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
Document Type: Research Article
Publication date: 2012-01-01
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites