Predicting and Proposed Control of Gordonia amarae Occurrence in an Activated Sludge Process and Physicochemical Factors in Plant Operation Related to the Foaming Event

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This is the first study to use qPCR to monitor for G. amarae which was detected approximately 2 months before the foaming event. This provides an early warning sign of this problematic bacterium. The study also indicates that RAS chlorination before the organisms begins branching is most important, and only molecular techniques can detect this growth phase. G. amarae growth was negatively associated with temperature and AOB and heterotrophic bacterial growth. Overall SRT and F/M were not associated with G. amarae population (R2 = 0.008 and 0.085 for SRT and F/M, respectively), but SRT was positively correlated with the growth period. cBOD to nBOD ratio had high R2 = 0.433. G. amarae were able to grow during a high carbon to nitrogen substrate ratio. HRT also had strong relationship to G. amarae population increases (R2 = 0.43), which supports the hypothesis that in this plant's higher G. amarae concentrations were observed when contact with readily available soluble carbon sources were present.

Keywords: Activated Sludge; Foaming; Gordonia amarae; quantitative Polymerase Chain Reaction

Document Type: Research Article


Publication date: January 1, 2011

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