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Respirometric Microbioreactors for Biokinetic Estimation of Nitrification Activity

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Microfluidics based techniques offer great promise to inspect and interrogate bacterial systems in terms of metabolic processes and kinetics. Herein, the potential for combining microfluidics and respirometry for the estimation of nitrification biokinetics was evaluated. Ammonia oxidation kinetics were determined in specially designed and fabricated microbioreactors. Using these microbioreactors, the impact of shear rate on the maximum specific growth rate (μmax) and half–saturation coefficient (Ks) were determined. At the shear rates tested (which are higher than those typically employed in engineered wastewater systems), μmax and Ks were both nearly invariable suggesting minimal mass transfer limitation. Notably, correlation between μmax and Ks was very low, suggesting a high degree of precision in the parameter estimates obtained.

Document Type: Research Article


Publication date: January 1, 2011

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