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Rapid Detection of Bacteria in Water by Quantum Dots Labeling and Magnetic Separation

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Wheat germ agglutinin functionalized CdSe-ZnS quantum dots (WGA-QDs) were utilized as the fluorescent label for the bacterial detection. The labeled bacteria cells were enriched and separated with SiMAG-PEI, a type of non-porous silica-encapsulated Fe3O4 magnetic particles with anion-exchange group. The study indicated that the bacterial cells interact strongly with WGA-QDs and SiMAG-PEI and the fluorescence intensity increases with increasing bacterial concentration within the range of 102–107 cfu/ml. This method can be used to detect total bacterial concentration in about 1.5 hour.
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Keywords: Bacterial detection; Nanotechnology; magnetic separation; operations and maintenance; quantum dots

Document Type: Research Article

Publication date: 2011-01-01

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