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Rapid Detection of Bacteria in Water by Quantum Dots Labeling and Magnetic Separation

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Abstract:

Wheat germ agglutinin functionalized CdSe-ZnS quantum dots (WGA-QDs) were utilized as the fluorescent label for the bacterial detection. The labeled bacteria cells were enriched and separated with SiMAG-PEI, a type of non-porous silica-encapsulated Fe3O4 magnetic particles with anion-exchange group. The study indicated that the bacterial cells interact strongly with WGA-QDs and SiMAG-PEI and the fluorescence intensity increases with increasing bacterial concentration within the range of 102–107 cfu/ml. This method can be used to detect total bacterial concentration in about 1.5 hour.

Keywords: Bacterial detection; Nanotechnology; magnetic separation; operations and maintenance; quantum dots

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864711802793416

Publication date: January 1, 2011

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  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

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