In Situ Identification and Quantification of Bioaugmentation Products in Wastewater Treatment Facilities
Bioaugmentation of wastewater treatment processes has gained greater industrial support recently. Until recently, there has been no way to quantify the presence and activity of the exogenous organisms added to the complex wastewater treatment communities. Current In Situ microbial identification methods are focused on 16S rDNA-based methods and include fluorescence In Situ hybridization (FISH) which offers insight into microbial community dynamics. However, this cannot be reliably used to distinguish between closely related organisms and lacks sensitivity. While end-point PCR is a sensitive alternative, it lacks the specificity needed to accurately identify individual microbial strains such as those added in bioaugmentation schemes. In order to identify and quantify a specific exogenous strain in complex communities, a combination of microbial strain-specific quantitative PCR (qPCR) and a recent advancement in FISH technologies, Recognition of INdividual Gene-FISH (RING-FISH), has been employed. The combination of techniques is highly specific, sensitive and quantitative and can be used to both monitor the presence and integration of bioaugmentation products in wastewater treatment.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: 01 January 2011
More about this publication?
- Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed. A subscription to the Proceedings of the Water Environment Federation includes access to most papers presented at the annual WEF Technical Exhibition and Conference (WEFTEC) and other conferences held since 2000. Subscription access begins 12 months after the event and is valid for 12 months from month of purchase. A subscription to the Proceedings of the Water Environment Federation is included in Water Environment Federation (WEF) membership.
WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access. Access begins 12 months after the conference or event
- Subscribe to this Title
- Membership Information
- About WEF Proceedings
- WEFTEC Conference Information
- Learn about the many other WEF member benefits and join today
- Ingenta Connect is not responsible for the content or availability of external websites