Skip to main content

In Situ Identification and Quantification of Bioaugmentation Products in Wastewater Treatment Facilities

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial

Abstract:

Bioaugmentation of wastewater treatment processes has gained greater industrial support recently. Until recently, there has been no way to quantify the presence and activity of the exogenous organisms added to the complex wastewater treatment communities. Current In Situ microbial identification methods are focused on 16S rDNA-based methods and include fluorescence In Situ hybridization (FISH) which offers insight into microbial community dynamics. However, this cannot be reliably used to distinguish between closely related organisms and lacks sensitivity. While end-point PCR is a sensitive alternative, it lacks the specificity needed to accurately identify individual microbial strains such as those added in bioaugmentation schemes. In order to identify and quantify a specific exogenous strain in complex communities, a combination of microbial strain-specific quantitative PCR (qPCR) and a recent advancement in FISH technologies, Recognition of INdividual Gene-FISH (RING-FISH), has been employed. The combination of techniques is highly specific, sensitive and quantitative and can be used to both monitor the presence and integration of bioaugmentation products in wastewater treatment.

Document Type: Research Article

DOI: https://doi.org/10.2175/193864711802793371

Publication date: 2011-01-01

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more