Skip to main content

Anammox Process Inhibition by Oxytetracycline: Short-and Long-Term Experiments and Model Evaluation

Buy Article:

$17.50 plus tax (Refund Policy)

Or sign up for a free trial


Batch experiments were conducted to investigate the effect of oxytetracycline on anaerobic ammonium oxidation (anammox) process. The short-and long-term effects on anammox activity were studied by measuring ammonium, nitrite, and nitrate concentrations over time. Experiments were conducted at ammonium and nitrite concentrations of 60–90 mg N/L and 60–190 mg N/L, respectively (ammonium:nitrite ratio from 1:1–1:2.25), oxytetracycline concentrations of 23–100 mg/L, and biomass concentrations of 300–800 mg/L. In the short-term study, anammox activity was inhibited by all oxytetracycline concentrations studied. However, daily addition of oxytetracycline to a concentration of 5±3.5 mg/L in the anammox sequencing batch reactor completely inhibited anammox activity in the fifth week. Fluorescent in situ hybridization was used to identify autotrophic ammonium oxidizing bacteria (Nitrosomonas spp., Nitrobacter spp., Nitrospira spp., Candidatus Brocadia anammoxidans, and Candidatus Kuenenia stuttgartiensis). The population of anammox culture was significantly decreased while Nitrosomonas spp. and Nitrospira spp. increased in the fifth week compared with the first week of experiment. A not-competitive model fit the anammmox inhibition data at oxytetracycline concentrations of 0–100 mg/L quite well with V max of 0.0435 mg N/mg VSS-hr and Ki of 54.66 mg/L.

Keywords: Anammox; Inhibition; Oxytetracycline; model evaluation

Document Type: Research Article


Publication date: January 1, 2011

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more
Real Time Web Analytics