Class B biosolids have been found to contain a wide variety of pathogens at detectable levels. Due to the complexity and expense of pathogen-specific microbiological assays, monitoring efforts need to be focused on those pathogens which present the greatest risk. However, the health
risk presented by a pathogen is a function of several different factors. First, the concentration of the pathogen typically found in biosolids, which directly influences the dose. Second, the dose response characteristics of the microbe, that is, how many microbes are required to produce a
specified probability of producing infection. The dose required for a 50% chance of infection can vary from fewer than ten to hundreds of thousands of organisms. A third factor is persistence in the environment, as the longer a microbe endures in the environment, the more opportunities for
exposure will accrue over time.
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