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Quantification of Selected Pathogens in Agricultural and Municipal Wastes

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Samples from three dairy farms and a publicly owned waste water treatment plant in northern New York were analyzed for enteric bacterial populations using real time q-PCR. Results indicate that dairy waste management processes like anaerobic digestion significantly reduce pathogens. It was observed that pathogen concentrations were correlated to sample type, moisture content, and volatile solids content of samples. Bedding solids harbored enteric bacteria irrespective of being organic or inorganic. However, bacterial concentrations in inorganic bedding samples were a log-order less than organic bedding samples. These correlations may be useful to dairy farm operators for monitoring pathogen reduction during manure management, thereby lowering the microbiological risks associated with recycle and reuse of solid residuals for bedding and applying manures of contaminated livestock to land.

Keywords: CAFO; anaerobic digestion; enteric bacteria; manure management; zoonotic pathogen

Document Type: Research Article


Publication date: 2010-01-01

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