Author: Ebitson, Michael

Source: Proceedings of the Water Environment Federation, WEFTEC 2010: Session 81 through Session 90 , pp. 6242-6247(6)

Publisher: Water Environment Federation

Abstract:

This automated solid phase extraction (SPE) method was developed to help streamline the extraction process for determining low levels of hormones in drinking water and wastewater. The SPE-DEX^® 4790 Automated Extractor System was used in this study, as well as the DryVap^® Concentrator System. The Agilent 1200SL HPLC with an API 4000 MS/MS was used to analyze the samples. The automated extractor was paired with the Atlantic™ hydrophilic-lipophilicbalanced (HLB) SPE Disks (47 mm). The Atlantic HLB disk was required to retain the low level 50 parts per trillion spiked amount of hormones in each 500 milliliter (mL) sample at 100 mL/minute. The selected hormones in this research were 17-alpha-estradiol, estrone, ethynyl, estradiol, progesterone, and testosterone. With the correct solvents selected for prewets (to condition the disk) and eluting (to extract analytes from the disk), the best results were obtained through optimizing the soak and air-dry times for the disk. The DryVap automated concentrator system proved an asset by separating the water from the solvent extract mixture through the use of a hydrophobic membrane disk. It also concentrated the extract and performed the required solvent exchange to methanol. The results of the research proved reproducible and provided excellent recoveries ranging from 79% - 96% for flow rates exceeding 100 mL/minute.

Keywords: Environmental; Water; Hormones; Solid Phase Extraction; Pharmaceutical; Automated Sample Preparation

Document Type: Research article

Publication date: 2010-01-01

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