Skip to main content

An Automated Detection Technology for On-Site E. coli and Coliform Bacteria Monitoring

The full text article is not available for purchase.

The publisher only permits individual articles to be downloaded by subscribers.

or click here to sign up for a free trial

Abstract:

We have developed a new detector for Escherichia coli and coliform bacteria that can be used on site with automated signal analysis. Fluorogenic “targeted substrates” and a fibre-optic detector monitor glucuronidase and galactosidase enzymes as indicators for E. coli and Total Coliform, respectively. The test has been certified by AOAC and approved for use in laboratories by the Ontario Ministry of the Environment. The test has been demonstrated on samples from drinking water to sewage, with no change in the analysis method and no need for dilutions. Single organisms were detected in about 13 h, with highly contaminated samples detected earlier. The bacteria were quantified using the continuous signal and a model for growth kinetics. Sample-to-sample repeatability was similar to plate-counting methods. The calibration function for a new site or type of bacteria can be adjusted using a small number of samples.

Keywords: Bacteria; Coliform; Drinking Water; E. coli; Enzyme Activity; Fibre-optic; Fluorescence

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864710798207675

Publication date: January 1, 2010

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • Ingenta Connect is not responsible for the content or availability of external websites
wef/wefproc/2010/00002010/00000008/art00013
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more