Skip to main content

Microbial Electrochemical Cells as a Research Tool to Probe Microbial and Biofilm Kinetics

Buy Article:

$17.50 plus tax (Refund Policy)

Abstract:

Anode-respiring bacteria (ARB) are known to produce electrical current from organic compounds and wastes in microbial electrochemical cells (MXCs). Recent kinetic experiments show that the current generated by ARB is not hindered by extracellular electron transfer; thus, it directly measures their respiration rate in real time. Given the high precision of electrical current measurements, determining the respiration rate of ARB gives scientists and engineers a new tool to study microbial and biofilm kinetics. We outline three experiments that were performed using electrochemical measurements. First, we tracked the growth of ARB in a biofilm to measure their specific growth rate (μ). Our results show an 18% decrease in μ as the biofilm developed. The capability to track respiration rates in the ARB biofilm over various orders of magnitude allows us to track these changes in growth during biofilm development. Second, we measured the rate of endogenous decay for an ARB biofilm by measuring the current generated with no electron donor. We tracked how this rate decreased with time over a 5-day period. After this period, extracellular polymeric substances (EPS) were significantly decreased within the biofilm, as shown by scanning electron microscopy. Third, we observed the kinetic response of an ARB biofilm after the addition of butyrate, a fermentable substrate. The current density increased within a few seconds of butyrate addition, suggesting that butyrate can partially be consumed directly by ARB. These experiments are examples of the electrochemical measurements that can be performed to probe microbial and biofilm kinetics.

Keywords: anode-respiring bacteria; electrochemistry; kinetics; microbial fuel cells

Document Type: Research Article

DOI: http://dx.doi.org/10.2175/193864710798208476

Publication date: January 1, 2010

More about this publication?
  • Proceedings of the Water Environment Federation is an archive of papers published in the proceedings of the annual Water Environment Federation® Technical Exhibition and Conference (WEFTEC® ) and specialty conferences held since the year 2000. These proceedings are not peer reviewed.

    WEF Members: Sign in (right panel) with your IngentaConnect user name and password to receive complimentary access.
  • Subscribe to this Title
  • Membership Information
  • About WEF Proceedings
  • WEFTEC Conference Information
  • ingentaconnect is not responsible for the content or availability of external websites
wef/wefproc/2010/00002010/00000007/art00004
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more